Simple stain and the gram stain in most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear (figure 1) in this procedure living, potentially pathogenic bacteria are smeared on the glass slide and allowed to air dry then the slide is heated so that the bacteria are. Gram negative unknown lab report essay sample abstract the gram stain is a differential stain that utilizes a primary stain of crystal violet (ie a purple dye), iodine, alcohol decolorization, and safranin (ie a tinted red dye) used as counterstain in order to determine the morphology of the bacteria a gram-stain followed the. Gram positive vs gram negative bacteria - gram staining technique between gram positive and gram negative bacteria we also look at the gram staining technique essay writing service: http. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents the gram stain procedure distinguishes between gram positive and gram negative groups by coloring these cells red or violet.
In a gram stain test, bacteria are washed with a decolorizing solution after being dyed with crystal violet on adding a counterstain such as safranin or fuchsine after washing, gram-negative bacteria are stained red or pink while gram-positive bacteria retain their crystal violet dye this is due. Gram’s staining the gram staining method is named after the danish bacteriologist hans christian gram (1853 – • although gram stain is useful in staining bacteria, certain fungi such as candida and cryptococcus are observed as gram positive yeasts. Exercise 10 gram stain gram staining, a differential staining technique, is one of the most important methods used by microbiologist the technique allows us to separate bacteria into two fundamental groups gram positive and gram negative.
Learn microbiology essay questions with free interactive flashcards choose from 122 different sets of microbiology essay questions flashcards on quizlet. Below is an essay on biology gram staining lab from anti essays, your source for research papers, essays, and term paper examples purpose: this lab is to test the difference in reaction of the bacteria’s cell wall to different chemicals. Gram staining of bacterial cells is neither an accurate nor elaborate technique, but nevertheless it is practically useful to distinguish two big domains of eubacteria species: gram-positive bacteria that include most of the firmicutes, and gram-negative bacteria that include the rest the structure of the flagellar basal body differs between.
Gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884 gram staining is still the cornerstone of bacterial identification and taxonomic division. Gram staining technique is the most important and widely used microbiological differential staining technique it categorizes bacteria according to their gram character (gram positive or gram negative) along with their staining characteristics, gram positive and gram negative bacteria differ from each other in various aspects which are listed below . Abstract the main objective of this lab was to identify different bacteria by simple, negative, and gram staining to view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. For example, a gram stain can be used to identify the shape of the microorganism and the number of peptidoglycan layers it has the shape can be and type of cell can be used to determine which genera the unknown bacteria could be classified under.
The word ‘gram-staining‘ technique arose from the danish bacteriologist christian gram in 1884this stain is a weak alkaline solution of a crystal violet though it is an old technique, still consider as the cornerstone in the field of microbiology for bacterial identification. Microbiology- identifying unknowns: micro lueteus essay 1185 words may 10th, 2016 5 pages show more will numair (using an individual colony from the streak plate) was the gram stain this was the test that ultimately eliminated all the other options other than m luteus, but 2 more tests were run for confirmation essay unknown. The bacteria that are stained by this method are called gram-positive, the others are gram-negative the distinction in the staining is related to a fundamental difference in the structure of cell wall and also a corresponding difference in their sensitivity to various chemical agents other than the stain.
The gram stain is a test used to identify bacteria by the composition of their cell walls it is named for hans christian gram, who developed the technique in 1884 it is named for hans christian. Gram-positive bacteria have a thick peptidoglycan layer, which gram-negative bacteria have an inner membrane, a thin peptidoglycan layer, and an outer membrane the outer membrane protects the gram-negative against against many antibiotics that couls otherwise damage the peptidoglycan layer or inner cell.
Bacterial classification, structure and function introduction the purpose of this lecture is to introduce you to terminology used in microbiology the lecture will: 1 cover different classification schemes for grouping bacteria, especially the use of gram positive bacteria stain blue-purple and gram negative bacteria stain red the. Gram staining lab introduction gram staining is a very important technique used in biology labs all over the world it is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Introduction: the gram staining of bacteria is a widely used method to identify unknown bacteria the purpose of this method is to find out identities of unidentified bacteria by using this tool we can group bacteria into two main groups: gram-positive and gram-negative. The stain will stick if the peptidoglycan layer is thick enough, meaning that all gram-positive bacteria will retain their stain (hence, they are gram-positive), whereas the crystal violet-iodine complex can slip past the thinner peptidoglycan layer of gram-negative bacteria.